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rabbit anti notch3 icd  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti notch3 icd
    C-terminal Notch activating mutations signal by alternative mechanisms. (A) Schematic diagram showing the <t>intracellular</t> <t>domain</t> of Drosophila WT Notch and mutant constructs used, PPxF, ΔPEST, and ANK. NB all constructs have intact ECD (not shown). (B) Overactivation of the mutant Notch constructs and WT+Dx, analyzed by NRE-luciferase assay in S2 cells. ***P < 0.001 compared to WT Notch. (C) Activation of WT Notch and mutant constructs with and without treatment of cells with BB-94, MβCD, or ML-SI1. *, **, and *** indicate P < 0.05, 0.01, and 0.001 by two-tailed t test, respectively for comparisons indicated on the graph. Error bars represent SEM. Sample sizes are indicated in figure. (D) Time course of colocalization between Notch ECD antibody uptake and Cav-1-mRFP. Notch ECD antibody was labeled for 15 min (pulse), then chased for 0, 10, 30, and 60 min, and the percentage of Notch ECD on Cav1-mRFP positive spots was scored with WT Notch, PPxF, or ANK expressing cells. Data from 3 experimental repeats, with 50–100 puncta scored per repeat, error bars SEM.
    Rabbit Anti Notch3 Icd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+notch3+icd/Notch3+Antibody/pmc10868400-289-13-18
    Average 94 stars, based on 182 article reviews
    rabbit anti notch3 icd - by Bioz Stars, 2026-10
    94/100 stars

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    1) Product Images from "Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains"

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.202211041

    C-terminal Notch activating mutations signal by alternative mechanisms. (A) Schematic diagram showing the intracellular domain of Drosophila WT Notch and mutant constructs used, PPxF, ΔPEST, and ANK. NB all constructs have intact ECD (not shown). (B) Overactivation of the mutant Notch constructs and WT+Dx, analyzed by NRE-luciferase assay in S2 cells. ***P < 0.001 compared to WT Notch. (C) Activation of WT Notch and mutant constructs with and without treatment of cells with BB-94, MβCD, or ML-SI1. *, **, and *** indicate P < 0.05, 0.01, and 0.001 by two-tailed t test, respectively for comparisons indicated on the graph. Error bars represent SEM. Sample sizes are indicated in figure. (D) Time course of colocalization between Notch ECD antibody uptake and Cav-1-mRFP. Notch ECD antibody was labeled for 15 min (pulse), then chased for 0, 10, 30, and 60 min, and the percentage of Notch ECD on Cav1-mRFP positive spots was scored with WT Notch, PPxF, or ANK expressing cells. Data from 3 experimental repeats, with 50–100 puncta scored per repeat, error bars SEM.
    Figure Legend Snippet: C-terminal Notch activating mutations signal by alternative mechanisms. (A) Schematic diagram showing the intracellular domain of Drosophila WT Notch and mutant constructs used, PPxF, ΔPEST, and ANK. NB all constructs have intact ECD (not shown). (B) Overactivation of the mutant Notch constructs and WT+Dx, analyzed by NRE-luciferase assay in S2 cells. ***P < 0.001 compared to WT Notch. (C) Activation of WT Notch and mutant constructs with and without treatment of cells with BB-94, MβCD, or ML-SI1. *, **, and *** indicate P < 0.05, 0.01, and 0.001 by two-tailed t test, respectively for comparisons indicated on the graph. Error bars represent SEM. Sample sizes are indicated in figure. (D) Time course of colocalization between Notch ECD antibody uptake and Cav-1-mRFP. Notch ECD antibody was labeled for 15 min (pulse), then chased for 0, 10, 30, and 60 min, and the percentage of Notch ECD on Cav1-mRFP positive spots was scored with WT Notch, PPxF, or ANK expressing cells. Data from 3 experimental repeats, with 50–100 puncta scored per repeat, error bars SEM.

    Techniques Used: Mutagenesis, Construct, Luciferase, Activation Assay, Two Tailed Test, Labeling, Expressing

    Related Articles

    Mutagenesis:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).

    Construct:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).

    Luciferase:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).

    Activation Assay:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).

    Two Tailed Test:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).

    Labeling:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).

    Expressing:

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains
    Article Snippet: The immunoprecipitated samples and 10% lysates were separated on NuPage 3–8% Tris-Acetate gels (Thermo Fisher Scientific) and transferred to PVDF membranes (Merck).



    Similar Products

    rabbit anti notch3 icd  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc rabbit anti notch3 icd
    C-terminal Notch activating mutations signal by alternative mechanisms. (A) Schematic diagram showing the <t>intracellular</t> <t>domain</t> of Drosophila WT Notch and mutant constructs used, PPxF, ΔPEST, and ANK. NB all constructs have intact ECD (not shown). (B) Overactivation of the mutant Notch constructs and WT+Dx, analyzed by NRE-luciferase assay in S2 cells. ***P < 0.001 compared to WT Notch. (C) Activation of WT Notch and mutant constructs with and without treatment of cells with BB-94, MβCD, or ML-SI1. *, **, and *** indicate P < 0.05, 0.01, and 0.001 by two-tailed t test, respectively for comparisons indicated on the graph. Error bars represent SEM. Sample sizes are indicated in figure. (D) Time course of colocalization between Notch ECD antibody uptake and Cav-1-mRFP. Notch ECD antibody was labeled for 15 min (pulse), then chased for 0, 10, 30, and 60 min, and the percentage of Notch ECD on Cav1-mRFP positive spots was scored with WT Notch, PPxF, or ANK expressing cells. Data from 3 experimental repeats, with 50–100 puncta scored per repeat, error bars SEM.
    Rabbit Anti Notch3 Icd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+notch3+icd/Notch3+Antibody/pmc10868400-289-13-18
    Average 94 stars, based on 1 article reviews
    rabbit anti notch3 icd - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    C-terminal Notch activating mutations signal by alternative mechanisms. (A) Schematic diagram showing the intracellular domain of Drosophila WT Notch and mutant constructs used, PPxF, ΔPEST, and ANK. NB all constructs have intact ECD (not shown). (B) Overactivation of the mutant Notch constructs and WT+Dx, analyzed by NRE-luciferase assay in S2 cells. ***P < 0.001 compared to WT Notch. (C) Activation of WT Notch and mutant constructs with and without treatment of cells with BB-94, MβCD, or ML-SI1. *, **, and *** indicate P < 0.05, 0.01, and 0.001 by two-tailed t test, respectively for comparisons indicated on the graph. Error bars represent SEM. Sample sizes are indicated in figure. (D) Time course of colocalization between Notch ECD antibody uptake and Cav-1-mRFP. Notch ECD antibody was labeled for 15 min (pulse), then chased for 0, 10, 30, and 60 min, and the percentage of Notch ECD on Cav1-mRFP positive spots was scored with WT Notch, PPxF, or ANK expressing cells. Data from 3 experimental repeats, with 50–100 puncta scored per repeat, error bars SEM.

    Journal: The Journal of Cell Biology

    Article Title: Alternative mechanisms of Notch activation by partitioning into distinct endosomal domains

    doi: 10.1083/jcb.202211041

    Figure Lengend Snippet: C-terminal Notch activating mutations signal by alternative mechanisms. (A) Schematic diagram showing the intracellular domain of Drosophila WT Notch and mutant constructs used, PPxF, ΔPEST, and ANK. NB all constructs have intact ECD (not shown). (B) Overactivation of the mutant Notch constructs and WT+Dx, analyzed by NRE-luciferase assay in S2 cells. ***P < 0.001 compared to WT Notch. (C) Activation of WT Notch and mutant constructs with and without treatment of cells with BB-94, MβCD, or ML-SI1. *, **, and *** indicate P < 0.05, 0.01, and 0.001 by two-tailed t test, respectively for comparisons indicated on the graph. Error bars represent SEM. Sample sizes are indicated in figure. (D) Time course of colocalization between Notch ECD antibody uptake and Cav-1-mRFP. Notch ECD antibody was labeled for 15 min (pulse), then chased for 0, 10, 30, and 60 min, and the percentage of Notch ECD on Cav1-mRFP positive spots was scored with WT Notch, PPxF, or ANK expressing cells. Data from 3 experimental repeats, with 50–100 puncta scored per repeat, error bars SEM.

    Article Snippet: Primary antibodies used in this study were sheep anti-NOTCH3 ECD (1:500; R&D systems), rabbit anti-NOTCH3 ICD (D11B8, 1:500; Cell Signaling Technology), mouse anti-EEA1 (E9Q6G, 1:200; Cell Signaling Technology), and rabbit anti-clathrin heavy chain (Cat# 4796, RRID:AB_10828486; Cell Signaling Technology, used 1:50).

    Techniques: Mutagenesis, Construct, Luciferase, Activation Assay, Two Tailed Test, Labeling, Expressing